Journal: Experimental Hematology

Article Title: Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies

doi: 10.1016/j.exphem.2017.09.011

Figure Lengend Snippet: Multiplexed SNP genotyping is an efficient method for genotyping BFU-E colonies . Number of colonies for which a genotype could be called out of 96 colonies for each mutation is shown. Five genes were genotyped by capillary sequencing and multiplexed SNP genotyping. Shaded regions highlight the number of colonies for which the same genotype was called by both technologies.

Article Snippet: The diluted amplified product was loaded onto the 192.24 Dynamic Array IFC for SNP Genotyping (BMK-M-192.24GT, Fluidigm) alongside a sample premixture including ROX reference dye and real-time master mixture.

Techniques: Mutagenesis, Sequencing

Journal: STAR Protocols

Article Title: Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

doi: 10.1016/j.xpro.2021.100641

Figure Lengend Snippet:

Article Snippet: Real-Time PCR of the 96.96 IFC (with the Biomark HD) and Quantification and data processing .

Techniques: Recombinant, Suspension, Saline, Binding Assay, Control, Multiplex Assay, Cell Isolation, Real-time Polymerase Chain Reaction, Software, Gene Expression, Transferring, Fluorescence, Microscopy